Can we produce a better wheat crop to feed the world?: Scientists uncover hidden wheat treasures

The wheat genome has been an enigma to scientists due to its exceptionally large and complex genome
The wheat genome has been an enigma to scientists due to its exceptionally large and complex genome

Scientists have uncovered a trove of hidden treasures in the wheat genome, which is hoped could improve yield quality and increase wider production of this critical food crop.

Entering a ‘wheat pan-genomics’ era from single to multiple wheat DNA references, scientists at the Earlham Institute (EI) aim to diversify one of the world’s most complex genomes to improve yield quality and increase wider production.

Bread wheat is the UK's most economically important crop and the world's most widely cultivated cereal, and wheat production is vital in both emerging and growing economies.

Therefore, understanding the genomics of wheat is essential to sustain increased yields for the growing global population, while protecting the crop from common disease epidemics and adaptation to extreme climate change conditions.

The wheat genome has been an enigma to scientists due to its exceptionally large and complex genome.

EI have now leveraged their genomics advances to work with both industry and research partners to enable the production of a better and more sustainable crop to aid global food security.

'The immediate incorporation of new technology into the breeding programme allows breeders to produce better varieties faster and cheaper'

Decipher complex genomes - the 'W2rap' tool

From the most sourced plant data reference released in November 2015, EI have developed a new method that is influencing industry and academia to further advance the field of wheat genomics into a multi-reference era.

The new wheat analyses came to fruition after delivery of the first wheat reference using the bioinformatics ‘w2rap’ tool for genome assembly developed by Bernardo Clavijo and his Algorithm Development Team at EI. Life science industry leaders Bayer Crop Sciences approached the team to run a pilot project sequencing a commercially significant wheat variety.

Now, the industry-academia partnership has resulted in a further four varieties being commissioned. A relationship that gives the industry access to unprecedented genome analysis power, while supporting the development of EI’s wheat genomics methods.

W2rap is a bioinformatics pipeline that can decipher complex genomes and produce robust assemblies to guide precision breeding. This genome assembly method will be applied to EI’s further wheat analysis, using tailored data generation based on work of the Institute’s Platforms and Pipelines Group at EI.

The unique tool will not only be able to assemble the wheat varieties sequence data but allow wheat genomes to be produced effectively, robustly and efficiently with next-generation sequencing technologies.

£2million grant

In parallel to this, a £2m grant awarded to EI in collaboration will aim to understand the genetic makeup of 14 different varieties of wheat cultivars important for global agriculture and analyse this data to help map the genetic variation in the crop.

This project includes the further sequencing and assembly of eight more wheat lines with the w2rap method, and will build wheat genetic resources for the purpose of crop breeding, preservation, and research.

Prof Neil Hall, Director of EI, said: “These new projects are extremely timely, as technical advances we have made in assembly and analysis of the wheat genome have overcome the hurdles in complexity and now enable us to approach wheat genetics in a whole new way.

“Now we should see the rapid advances in breeding programs that have previously been possible in other crops.”

Public wheat initiatives

Bernardo Clavijo, Bioinformatics Algorithms Project Leader, said: “w2rap will be of great value for public wheat initiatives; helping us to advance into wheat pan-genomics faster, and from a completely open and transparent public-research focus.

“From a breeder’s perspective, this is also a tool that allows them to peek inside their germplasm with unprecedented detail and robustness - allowing cross-analysis with our high-quality public wheat reference.

“In terms of global food security, the gains are twofold. The immediate incorporation of new technology into the breeding programme allows breeders to produce better varieties faster and cheaper.

“As well as the methods we develop enabling a fast transition from a single-reference to a many-genomes scenario that will effectively start to tap in the enormous variation of the world’s wheat genomic resources.”